Laboratory Manual for Biomedical Research
New Edition
Chapter 1. General Lab Techniques
Lab security and basic techniques
Advanced lab skills
Advanced lab skills (2)
Chapter 2. Molecular Biology -- Molecular Separation
Molecular Separation
DNA Agarose Gel Electrophoresis
RNA Agarose Gel Electrophoresis
SDS-Page - Polyacrylamide gel electrophoresis
2D Page-Two - Dimensional Polyacrylamide Gel Electrophoresis
Ion Exchange Chromatography
Gel Filtration Chromatography
Affinity chromatography
Chapter 3. Molecular Biology -- DNA and RNA
Nucleic acid methods (1)
Nucleic acid methods (2)
DNA isolation & related protocols
DNA Purification (glass milk vs electroelution)
DNA, RNA Sequencing
RNA Isolation and Purification
Isolation of DNA,RNA, and Protein simultaneously.
DNA mutation detection by SSCP
Preparation of DNA and RNA probes
Southern blot hybridization
Northern blot hybridization
Loss of Heterozygosity (LOH)
Gene knockout protocol
SiRNA gene knockout
Plasmid and its usefulness
DNA library construction
Microarray protocols.
Basic knowledge of microarray.
Introduction to Microarray.
MicroArray Procedure
Total RNA Isolation from cultured cells.
DNase Treatment of Total RNA
Making the single strand cDNA probe.
Automated Slide Processor (ASP) Version for hybridization.
Washing microarrays in ASP.
Processing of Array slide
Pre-hybridization of the processed slides (NON-Automated version).
Hybridization of Cy3 + Cy5 probe to glass array (NON-Automated version).
Preparation of Dendrimer Cy3 and Cy5.
Washing unbound probe from glass array (NON-Automated version).
Hybridization of Dendrimers (Cy3 and Cy5) to Array (NON-Automated version).
Washing unbound dendrimer from glass array(NON-Automated version).
Microarray Dababases
Troubleshooting
Other Microarray Protocols (1,2)
Gene transfection
Gene therapy for cancer
Molecular cloning
Conditional gene transfection(Tet on/off)
Chapter 4. Genetics
Epigenetics protocols
Mutagenesis protocols
Single nucleotide polymorphisms (SNPs)
Chapter 5. Molecular Biology --PCR Serials
PCR,RT-PCR,Real time PCR etc.
PCR (General Procedure)
Recommended Reagent Concentrations
Recommended Reaction Conditions
Initial Conditions
Temperature Cycling
"Hot Start" PCR
Asymmetric PCR for ssDNA Production
Detecting Products
Labelling PCR Products with Digoxigenin
Cleaning PCR Products
Sequencing PCR Products
Cloning PCR Products
AND ALWAYS REMEMBER
PCR Primer Design Tools
RT-PCR
Real time PCR
More PCR Protocols Online
Video and Animation of PCR
Mouse Genotyping by PCR
PCR Based Molecular Cloning
PCR Troubleshootings
Chapter 6. Molecular Biology – Protein
Protein methods
Protein labeling techniques
Protein sequencing
Subcellular fractionations
Western blot hybridization
Western Blot Hybridization Center
Western Blot Full Procedure (from Pierce)
Western Blotting Procedure with Video Show
Protocol 1: Western Blot
Sample protein preparation
Protein concentration assay
Electrophoresis and blotting
Blocking non-specific antigen
Incubation with primary antibody
Incubation with secondary antibody
Substrate (ECL) incubation
Restore a western blot using pierce stripping buffer
Western blot related solutions and buffers
Western blot handbook
Western blot troubleshooting
Protocol 2: Western Blot 2.
Protocol 3: Western Blot 3.
More online Western blot protocols (1) (2)
Protein chips
Kinase assay
Methods for detecting protein phosphorylation
Chapter 7. DNA Protein Interactions
EMSA
ChIP assay
Chapter 8. Immunohistochemistry/immunology
Immunology/Histology
Preparing silanized (+plus) slides
Microscopy Techniques
Confocal microscope technique
Electron microscopy
HE staining
DAPI & PI nucleic acid stain
Hybridization in situ
FISH
Special cell & cell fraction stains
Antibody purification
Antibody storage and handling
Conjugation of monoclonal antibodies
Antigen retrieval
Immunoperoxidase staining techniques
Immunofluorohistochemistry
Immunoprecipitation
Histotechnology--technical methods
FRQs for histochemistry
Laser Capture Microdissection
Elisa
Chapter 9. Cellular Biology
General cell culture protocols
Chromosome karyotype
Proliferation assays (MTT, BrdU, 3H-Thymidine incoporation)
Cell cycle assay
Migration assay
Stem cell & related protocols
Introduction
What are the unique properties of all stem cells?
What are embryonic stem cells?
What are adult stem cells?
What are the similarities and differences between embryonic and adult stem cells?
What are the potential uses of human stem cells and the obstacles that must be overcome before these potential uses will be realized?
Where can I get more information?
International Society for Stem Cell Research (ISSCR)
Culture of Human Embryonic Stem Cells (hESC)
Establishment and characterization of human embryonic stem cell line
Rat stem cell isolation and culture
Mouse stem cell isolation and culture
Induction of Stem Cell Differentiation
Cancer stem cells
Video Data on Stem Cell Research (click to show)
Apoptosis and related protocols
Soft Agar Assay for Colony Formation
Aorta ring assay
GFP transfection
Blood cell fractionation (white blood cell isolation)
Endothelial cell isolation and culture protocols
Flow cytometry (FCM)
Chapter 10. Metabolism, GC/MS, NMR and Proteomics
GC/MS Background
Glucose metabolism and its related protocols
PAS staining
Deoxyribose procedure
Ribose metabolism analysis
Polysaccharide sequencing
Lactate cleanup and derivative
Amino acids
Fatty acids
Lipid protocols
Cholesterol
Bile acids
Urea procedure
Choline Incorporation Assay
Isotope Ratio Mass Spectrometer (IRMS)
Liquid chromatography / mass spectrometer (LC/MS)
Proteomics
NMR protocols and tutorials
Chapter 11. Animal Experiments
Blood sampling from animals
Basic skills for animal experiments
Anatomy and physiology of animals
Cancer xenograft animal models
Transgenic animal procedures
Transgenic cancer models
Animal models for depression-like and anxiety-like behavior
Chapter 12. Worm: C. Elegans
C. Elegans Protocols
Chapter 13. HPLC and TLC
HPLC protocols
TLC
Chapter 14. Buffers and Solutions in Lab.
Commonly Used Buffer and solution (114 formats in alphabeta)
Compound solutions (Practical Molecular Biology)How to Make Simple Solutions and Dilutions (Department of Biology, Bates College)Reto's Buffers&Media Book (Private Page)Salt solutions(Practical Molecular Biology)Single Component Solutions (Practical Molecular Biology)Buffers (Gerard R. Lazo)
Antibiotics (Gerard R. Lazo)Buffer Listing (Salmon Lab.)
Cell Culture Media and Solutions (Donis-Keller lab)
Commonly used solutions (1)--- 1 ) 2 ) 3 ) 4 ) 5 ) 6 )
Commonly used solutions (2)--- 1 ) 2 ) 3 )
Solutions for molecular cloning---1 ) 2 ) 3 ) 4 ) 5 ) 6 ) 7 ) 8 ) 9 )
Chapter 15. Other Resources
Free eBooks at Library Online
Cinema Online, Free Movies --(1) (2) (3)
Progresses in Life Science
Progress in Cancer Apoptosis -- (1) (2) (3)
Progress in Gene Therapy --- (1) (2) (3)
Progress in Neuronal Regeneration -- (1) (2) (3)
Progress in AIDS Treatment -- (1) (2) (3)
Progress in Organ Transplantation -- (1) (2) (3)
Progress in Stem Cell Research -- (1) (2) (3)
Free eBooks in life science (1)
Free eBooks in life science (2,3)
Most Updated Biomedical Books
Pathway databases
Pathway Search
Biomedical Job Opportunities
Tools for Statistics
Free Software pDRAW32 to Draw DNA Analysis Charts
Biological Educational Resources
Genetics Education
Resources of Medical Biochemistry
Textbooks and Lab Manuals
Sunday, May 4, 2008
DNase Footprinting
Solid phase DNase I footprinting: quick and versatile
Solid phase DNase I footprinting: quick and versatile. Raphael Sandaltzopoulos and Peter B.Becker*. European Molecular Biology Laboratory, Gene Expression ...
nar.oxfordjournals.org/cgi/reprint/22/8/1511.pdf
DNase I Footprinting DNase I Footprinting Title | Overview ...
DNase I Footprinting. Title | Overview | Procedure | Solutions | BioChemicals | Hints | Printable Version. Overview ...
www.koko.gov.my/CocoaBioTech/Protein%20DNA5.html
Springer Protocols: Abstract: DNase i footprinting using PCR ...
Springer Protocols is the largest subscription-based electronic database of reproducible laboratory protocols in the Life and Biomedical Sciences.
www.springerprotocols.com/Abstract/doi/10.1385/0-89603-288-4:379
Springer Protocols: Abstract: DNase I Footprinting
Low C. M. L., Olsen R. K., and Waring M. J. (1984) Sequence preferences in the binding to DNA of triosim A and TANDEM as reported by DNase I footprinting. ...
www.springerprotocols.com/Abstract/doi/10.1385/0-89603-447-X:1
Solid-phase DNase I Footprinting
4 [ 1 9 9 5 ]. 25. CONTENTS. Introduction. Solid-phase techniques have diversified. the repertoire of tools in molecular biology ...
www.roche-applied-science.com/PROD_INF/BIOCHEMI/No.4_95/p25-27.pdf
Footprinting in the 21st century Chromatin preparation and DNase I ...
Footprinting in the 21st century. Chromatin preparation and DNase I treatment. Summary. Cells are spun down, the supernatant removed, the pellet resuspended ...
science.cancerresearchuk.org/sci/chromrep/pdf/footprint.pdf?version=1
Chapter 16: Protease Footprinting (PDF)
INTRODUCTION, 285. Advantages and limitations, 287. Types of proteolysis, 288. Alternative methods, 289. OUTLINE OF PROCEDURE, 290. End labeling, 290 ...
www.proteinsandproteomics.org/content/free/protocols_2/285-312-PI-Ch16.pdf
Biochemistry 611 Nucleic Acids 8-28-07
Biochemistry 611 Nucleic Acids 8-28-07.
www.mc.uky.edu/biochemistry/courses/bch611/2007/wilkerson/Bioch611_8_28_2007.ppt
DNase I-Primer Extension Footprinting Of Chromatin Assembled On Template DNA
Mapping DNase-I-Hypersensitive Sites (Subscription Required)
Mapping Protein-Binding Sites On DNA By DNase I Footprinting - Subscription Required
Preparation Of End-Labeled DNA Probes By Conventional Kinase For DNA Footprinting Analysis
DNase Footprinting (1)
Core Footprinting System Technical Bulletin TB137
T e ch ni cal Bu llet in. Core Footprinting System. INSTRUCTIONS FOR USE OF PRODUCT E3730. PRINTED IN
www.promega.com/tbs/tb137/tb137.pdf
Catharineís Footprint Protocol
Catharineís Footprint Protocol. August, 1999. This protocol assumes that the user has already performed gel-shift experiments with their fragment and ...
www.stanford.edu/~ceastman/footprint.html
DNase I footprinting. P.J. Shaw pjs14 at le.ac.uk Sat Jul 26 04:12:40 EST 1997. Previous message: DNase I footprinting; Next message: QIAQuick and QIAPrep
www.bio.net/bionet/mm/methods/1997-July/059706.html
staff.aist.go.jp/m.machida/technicalnote.htm
Mapping Protein-binding Sites on DNA by DNase I Footprinting ...
Search CSH Protocols. Advanced Search · Find Protocols · Find a Kit · Access Personal Page · Submit Protocols · Help · Subscribe · About CSH Protocols ...
www.cshprotocols.org/cgi/content/full/2006/2/pdb.prot3947
1. DNase I Footprinting. Keith R. Fox. 1. Introduction. Footprinting provides a simple, quick, and reasonably inexpensive method ...
www.springerlink.com/index/tv748uh685874403.pdf
Analysis of Protein-DNA Interactions
The characterization of the protein-DNA interactions usually requires three levels of analysis:. GENETIC. determination of the nucleotide sequence of the ...
www.mun.ca/biochem/courses/4103/topics/protein_DNA.html
DNA Footprinting with an automated DNA Analyzer: does the shoe fit?
DNA Footprinting with an. automated DNA Analyzer:. does the shoe fit? Michael Zianni. Manager, Plant-Microbe Genomics Facility ...
www.abrf.org/ResearchGroups/DNASequencing/Activities/DNAFootprinting.pdf
Core Footprinting System Technical Bulletin TB137
T e ch ni cal Bu llet in. Core Footprinting System. INSTRUCTIONS FOR USE OF PRODUCT E3730. PRINTED IN
www.promega.com/tbs/tb137/tb137.pdf
DNase I footprinting. P.J. Shaw pjs14 at le.ac.uk Sat Jul 26 04:12:40 EST 1997. Previous message: DNase I footprinting; Next message: QIAQuick and QIAPrep
www.bio.net/bionet/mm/methods/1997-July/059706.html
Protocol F.2. DNaseI Footprintint. Solutions. 10X Binding Buffer. 200 mM Tris 8.0 200 ml 1M Tris pH 8.0. 500 mM NaCl 100 ml 5M NaCl ...
genetics.med.harvard.edu/~cepko/protocol/mike/F2.html
staff.aist.go.jp/m.machida/technicalnote.htm
1. DNase I Footprinting. Keith R. Fox. 1. Introduction. Footprinting provides a simple, quick, and reasonably inexpensive method ...
www.springerlink.com/index/tv748uh685874403.pdf
Analysis of Protein-DNA Interactions DNA Footprinting with an automated DNA Analyzer: does the shoe fit?
DNA Footprinting with an. automated DNA Analyzer:. does the shoe fit? Michael Zianni. Manager, Plant-Microbe Genomics Facility ...
www.abrf.org/ResearchGroups/DNASequencing/Activities/DNAFootprinting.pdf
Tsukiyama Lab/protocols/DNase_I_Footprinting. 1. DNase I footprinting. I. Labeling of probe. •. 5 pmoles of primer #1. •. 1.5 µl of 10 X T4 PNK buffer ...
www.fhcrc.org/science/labs/tsukiyama/protocols/dnase_i_footprinting.pdf
A 96-well DNase I footprinting screen for drug–DNA interactions
Supplementary Material. PDF (6.1M) · Contents · Archive. Related material:. PubMed record, PubMed related arts, PubMed LinkOut, Substance, Taxonomy ...
www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1919508
A modified protocol for in vivo footprinting by ligation-mediated ...
Selected References. Page Browse · PDF (545K) · Contents · Archive. Related material:. PubMed record, PubMed related arts, PubMed LinkOut, Substance ...
www.pubmedcentral.nih.gov/articlerender.fcgi?artid=523615
Using a comparative in vivo DNase I footprinting technique to ...
J BIOCHEM MOLECULAR TOXICOLOGY. Volume 21, Number 5, 2007. Using a Comparative In Vivo DNase I Footprinting. Technique to Analyze Changes in Protein–DNA ...
doi.wiley.com/10.1002/jbt.20192
DMS Footprinting Protocols
DMS Footprinting Protocols
- Overview – Slide Shows for Footprinting
Chromatin Immunoprecipitation (2)
Other ChIP Online Resources
Chromatin Immunoprecipitation (ChIP) Online Resources
Chromatin immunoprecipitation - Wikipedia, the free encyclopedia
Chromatin Immunoprecipitation (ChIP) Assay
Chromatin Immunoprecipitation (ChIPs) Articles
Chromatin Immunoprecipitation and Microarray-Based Analysis of Protein Location
Chromatin Immunoprecipitation (ChIPs) Protocol (Farnham Lab)
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues
Chromatin immunoprecipitation on native chromatin from cells and tissues
Chromatin Immunoprecipitation Protocol for Histone Modification & Chromatin Associated Proteins
Chromatin Immunoprecipitation Protocol to Analyze Histone Modifications in Arabidopsis thaliana
chIP on chip.org Hosted by Bing Ren's lab at UCSD.
Chromatin Immunoprecipitation Protocol
Chromatin Immunoprecipitation Supplies
Comparison of Sample Preparation Methods for ChIP-chip Assays
NATIVE CHROMATIN IMMUNOPRECIPITATION (N-ChIP) PROTOCOL
High Throughput Screening of Chromatin Immunoprecipitates Using CpG-Island Microarrays
Use of Chromatin Immunoprecipitation to Clone Novel E2F Target Promoters
WGA Publication Supplemental Information
Whole Genome Amplification Protocol
Protocol for the fast chromatin immunoprecipitation (ChIP) method.
Workshop Chromatin Immunoprecipitation Protocol
Q ChIP - quick and quantitative chromatin immunoprecipitation
Immunoprecipitation (IP)/Chromatin Immunoprecipitation (ChIP Assay ...
Chromatin IP Chromatin Immunoprecipitation ChIP-IT
CSH Protocols -- Collected Resources : Immunoprecipitation
Application Note, ChIP-on-chip Gene Regulation
MPromDb: an integrated resource for annotation and visualization ...
Assessment of sera for chromatin-immunoprecipitation | Archive ...
Application of the Chromatin Immunoprecipitation Method to ...
ChIP (Chromatin Immunoprecipitation): Frequently Asked Questions
GEArray Analysis—ChIP-qPCR
Cavalli lab - Inserm workshop on ChIP and Immuno-FISH, June 2001-bis
ChIP ASSAY GUIDELINES AND CHECKPOINTS--Affymetrix
Chromatin Immunoprecipitation (1)
What is ChIP?
The principle of ChIP is simple: the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognise a protein or protein modification of interest can be used to determine the relative abundance of that antigen at one or more locations in the genome.
ChIP consists of the following steps: 1. Isolation of total chromatin (which may require cross-linking to fix the antigen of interest to its chromatin binding site) 2. Fragmentation of the chromatin (to achieve resolution) 3. Immunoprecipitation of the resulting chromatin fragments 4. Analysis of the immunoprecipitated fraction to determine the level of a target DNA sequence (or sequences) relative to its abundance in the input chromatin. The final analysis is typically carried out using PCR or hybridisation-based techniques. A comprehensive review of the procedures and methodologies of ChIP can be found in Allis & Wu (2004).
Protocols—for Chip on Chip
Protocols used in ChIP-on-chip experiments. Listed here are collections of RenLab protocols as well as protocals requested from other labs. ...
www.chiponchip.org/protocol.html
Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping ...
This protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo ...www.cshprotocols.org/cgi/content/full/2006/23/pdb.prot4560
Epigenome NoE - protocol: Chromatin Immunoprecipitation (ChIP) Assay
... and Copyright Information Contact Us. © 2004-2009 Epigenome Network of Excellence Printed from: http://www.epigenome-noe.net/researchtools/protocol.php.
www.epigenome-noe.net/researchtools/protocol.php?protid=10 Paul's CHIP—ChIP Protocol for Yeast
Mirmira Lab ChIP Protocol. Updated 12/3/02. Reagents:. ChIP sonication Buffer (1% .... Resuspend Protein A/G agarose so that it forms a uniform suspension. ...
faculty.virginia.edu/mirmira/resources_files/Protocols/MirmiraLabChIPprotocol.htm
ChIP Protocol - Oxford Gene Technology
Custom Microarray Services » ChIP Zone » ChIP Protocol ... DNA:protein complexes immunoprecipitated using an antibody to the protein of interest ...
www.ogt.co.uk/cms-chip-protocol.htm
Protocols- DNA Protocols > Chromatin Immunoprecipitation Protocols
ChIP assay protocol with 2 steps: in vivo formaldehyde cross-linking of whole cells and protein-protein and protein-DNA interactions, ...
www.molecularstation.com/protocol-links/DNA-Protocols/Chromatin-Immunoprecipitation-Protocols/
ChIP Protocol for X.laevis Lens1/FoxE3 Enhancer
ChIP protocol for X. laevis Lens1/FoxE3 enhancer Hajime Ogino 10/1/2007 .... (EZ ChIP kit) and incubate at 65°C for overnight to reverse the DNA-protein ...
faculty.virginia.edu/xtropicalis/chIP_analysis.htm
ChIP Protocol. Wende/Fong/Duncan. Kelly Lab. Revised May 29, 2007. DAY ONE (for cells): ... Keep at RT until protocol directs to put on ice. ...
kellylab.wustl.edu/protocols/ChIP_Protocol5-29-07.pdf –
Chromatin Immunoprecipitation (ChIP) Assay Protocol
(ChIP) Assay Protocol. Can be prepared in advance and stored at -80º ... ChIP Protocol. Page 2 of 12. Updated 6/30/06 By Heather N. Witt ...
www.genomecenter.ucdavis.edu/farnham/pdf/FarnhamLabChIP%20Protocol.pdf
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab). This protocol is based upon protocols from Mark Biggin, Dave Allis and Richard ...
genomecenter.ucdavis.edu/farnham/farnham/protocols/tissues.html
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION (X-ChIP) PROTOCOL
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION. (X-ChIP) PROTOCOL. Cross-linking and Cell Harvesting. 1. Start with 2 large dishes when confluent (1x10 ...
www.cosmobio.co.jp/product/kiki/pdf/ACM_chip_protocol.pdf
Day-and-a-half and two-and-a-half day ChIP
http://blog-di-j.blogspot.com/2007/12/optimized-chip-protocols.html. The blog post will also contain the most recent version of this protocol. 1.1 Protocol ...
www.jeremiahfaith.com/blog_figs/new_protocol_v1.0.pdf
Original ChIP protocol. Jeremiah Faith. Bioinformatics Program.
www.jeremiahfaith.com/blog_figs/original_protocol.pdf
1 BISHOP LAB DMC1 CHIP PROTOCOL Note: This protocol is optimized ...
BISHOP LAB DMC1 CHIP PROTOCOL. Note: This protocol is optimized for meiotic yeast cells sporulating in liquid. medium. CROSS-LINKING ...
bishoplab.bsd.uchicago.edu/protocols/BishopLabDmc1pChIP.pdf
ChIP Assay Protocol. By Michelle Kallesen, PhD (7/01). Formaldehyde cross-linking and chromatin immunoprecipitation assays of ...
www.bcm.edu/rosenlab/protocols/ChIP.pdf
Magnetic beads enable a streamlined ChIP method | Protocol Guide ...
We have developed a greatly simplified ChIP protocol that utilizes magnetic protein G-coated beads for antibody capture. This dramatically reduces washing ...
www.biotechniques.com/default.asp?page=protocol&subsection=article_display&id=112342
Steven Vokes McMahon Laboratory EB ChIP Protocol
EB ChIP Protocol. This protocol is a combination of Odom and colleagues (Odom et al., 2004) with a. Maniatis lab protocol (Daniel Smith and Diana Saville ...
www.people.fas.harvard.edu/~svokes/Vokes_ChIP_EB.pdf
1 ChIP protocol for procyclic forms 1. grow cells to a density of ...
ChIP protocol for procyclic forms. 1. grow cells to a density of 2 x 10. 7. cells/ml in SDM-79. 2. spin down (2000g for 10 min) and resuspend in 40 ml SDM- ...
tryps.rockefeller.edu/Protocols/ChIP_cj.pdf
Chromatin immunoprecipitation (ChIP) protocol from Drosophila embryos
Chromatin immunoprecipitation (ChIP) protocol from. Drosophila embryos. Overview. Target DNA binding protein that has been cross-linked to chromatin (which ...
www.flychip.org.uk/protocols/chip/purification_chromatin.pdf
Mapping DNA target sites of chromatin-associated proteins by ...
1. Reference: Cavalli, G., orlando, V and Paro, R. (1999). Mapping DNA target sites of chromatin-. associated proteins by formaldehyde cross-linking in ...
www.igh.cnrs.fr/equip/cavalli/Lab%20Protocols/FormaldehydeCrosslinking.pdf -
OneDay ChIP Kit—Product Manual
OneDay ChIP Kit Results. Troubleshooting Guide. Additional Protocols (Chromatin Shearing methods). References. Ordering Information ...
www.nippongene.com/pdf/man_oneday_english070405.pdf
DNA methylation detection using ChIP-GLAS
This short protocol is for the preparation of tissue samples that will be used in. ASB’s ChIP-GLAS system. You may use it to replace the corresponding part ...
www.avivasysbio.com/corp/Tissue%20ChIP%20protocol.PDF
Biochemistry and Molecular Genetics - UCHSC--ChIP for Yeast
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST. This protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. ...
www.uchsc.edu/sm/bbgn/ChIP_Protocol.htm
Chromatin Immunoprecipitation (ChIP) Methods, Techniques, Protocols
ChIP Protocol for Yeast - This protocol is derived from a paper by Miriam ... Chromatin IP (CHIP assay) - This protocol has some minor modification to the ...
www.antibodybeyond.com/applications/chip.htm
chromatin immunoprecipitation (ChIP) DNA. However, this method requires ..... Also see the troubleshooting guide of your ChIP preparation protocol or kit to ...
www.biomol.de/details/SA/chipqpcrmanual.pdf
Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from ...
Here, we describe how ChIP can be performed on non-fixed chromatin from animal ..... See Troubleshooting. DNA Extraction from Precipitated Chromatin (3 h) ...
www.cshprotocols.org/cgi/content/full/2007/12/pdb.prot4767
Sugden Lab. Last Modified 05/03/05 ChIP Protocol for 05/01/05-05 ...
ChIP Protocol for 05/01/05-05/03/05. Samples: Total, No Ab, IgG, E2F4, EBNA1, ORC2, ORC3. Sun 05/01/05. 1. Thaw 2 100ul-aliquots of Staph A cells (stored in ...
mcardle.oncology.wisc.edu/sugden/protocols/ChIP%20assay.pdf
Chromatin Immunoprecipation (ChIP) assay for 3T3-L1 preadipocytes
Chromatin Immunoprecipation (ChIP) assay for 3T3-L1 preadipocytes ... More troubleshooting will be required. But use this as a starting pointÉ ...
www-personal.umich.edu/~macdouga/Protocols/Chromatin%20Immunoprecipation.htm
next step of the protocol. The ChIP-IT Express Kit includes all .... In addition, the simplified ChIP-IT Express protocol enabled ChIP to be performed ...
www.biolynx.ca/Tech/Articles/ChIP%20protocol.pdf
Chromatin IP Chromatin Immunoprecipitation ChIP-IT
Using our chromatin and ChIP-IT reagents, the only variable in your ChIP will be the ... ChIP-IT Kits, simplify the task of troubleshooting your experiment, ...
www.activemotif.com/catalog/nuc_func/chipit/express
The principle of ChIP is simple: the selective enrichment of a chromatin fraction ... which we hope will help you troubleshoot this powerful method. ...
www.abcam.com/index.html?rid=310
More ChIP Tips (November 2005) | Abcam
Buying and contact info. Choose country,
www.abcam.com/index.html?pageconfig=resource&rid=10347&pid=5
troubleshooting with Chromatin Ip
troubleshooting with Chromatin Ip. Shwetal Mehta shwetal at mail.utexas.edu ... Hi, I have been having this problem with my ChIP assays (chromatin IP) of ...
www.bio.net/hypermail/yeast/2002-March/010125.html
Table 1 : Protocol for the fast chromatin immunoprecipitation ...--Troubleshooting
Protocol for the fast chromatin immunoprecipitation (ChIP) method. Joel D Nelson, Oleg Denisenko and Karol Bomsztyk ... Table 1. Troubleshooting table ...
www.nature.com/nprot/journal/v1/n1/fig_tab/nprot.2006.27_T1.html
The Science Advisory Board - Protocols, Product Reviews, Member ...
Yeast Chromatin Immunoprecipitation. Posted on Tuesday, November 18, 2003 ... Resuspend cell pellets in 400 ul ChIP lysis buffer+protease inhibitors ...
www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=223&criteria
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION (X-ChIP) PROTOCOL
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION. (X-ChIP) PROTOCOL ... Reverse cross-links by adding either 2µl RNase A (0.5mg/ml) when purifying DNA using ...
www.cosmobio.co.jp/product/kiki/pdf/ACM_chip_protocol.pdf
Chromatin Immunoprecipitation (ChIP) Assay Protocol. (Double Cross
www.bioinfo.utmb.edu/Brasier_Lab/protocols/ChIP.pdf
Subscribe to:
Posts (Atom)
Posts
