What is ChIP?
The principle of ChIP is simple: the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognise a protein or protein modification of interest can be used to determine the relative abundance of that antigen at one or more locations in the genome.
ChIP consists of the following steps: 1. Isolation of total chromatin (which may require cross-linking to fix the antigen of interest to its chromatin binding site) 2. Fragmentation of the chromatin (to achieve resolution) 3. Immunoprecipitation of the resulting chromatin fragments 4. Analysis of the immunoprecipitated fraction to determine the level of a target DNA sequence (or sequences) relative to its abundance in the input chromatin. The final analysis is typically carried out using PCR or hybridisation-based techniques. A comprehensive review of the procedures and methodologies of ChIP can be found in Allis & Wu (2004).
Protocols—for Chip on Chip
Protocols used in ChIP-on-chip experiments. Listed here are collections of RenLab protocols as well as protocals requested from other labs. ...
www.chiponchip.org/protocol.html
Chromatin Immunoprecipitation (ChIP) of Protein Complexes: Mapping ...
This protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo ...www.cshprotocols.org/cgi/content/full/2006/23/pdb.prot4560
Epigenome NoE - protocol: Chromatin Immunoprecipitation (ChIP) Assay
... and Copyright Information Contact Us. © 2004-2009 Epigenome Network of Excellence Printed from: http://www.epigenome-noe.net/researchtools/protocol.php.
www.epigenome-noe.net/researchtools/protocol.php?protid=10 Paul's CHIP—ChIP Protocol for Yeast
Mirmira Lab ChIP Protocol. Updated 12/3/02. Reagents:. ChIP sonication Buffer (1% .... Resuspend Protein A/G agarose so that it forms a uniform suspension. ...
faculty.virginia.edu/mirmira/resources_files/Protocols/MirmiraLabChIPprotocol.htm
ChIP Protocol - Oxford Gene Technology
Custom Microarray Services » ChIP Zone » ChIP Protocol ... DNA:protein complexes immunoprecipitated using an antibody to the protein of interest ...
www.ogt.co.uk/cms-chip-protocol.htm
Protocols- DNA Protocols > Chromatin Immunoprecipitation Protocols
ChIP assay protocol with 2 steps: in vivo formaldehyde cross-linking of whole cells and protein-protein and protein-DNA interactions, ...
www.molecularstation.com/protocol-links/DNA-Protocols/Chromatin-Immunoprecipitation-Protocols/
ChIP Protocol for X.laevis Lens1/FoxE3 Enhancer
ChIP protocol for X. laevis Lens1/FoxE3 enhancer Hajime Ogino 10/1/2007 .... (EZ ChIP kit) and incubate at 65°C for overnight to reverse the DNA-protein ...
faculty.virginia.edu/xtropicalis/chIP_analysis.htm
ChIP Protocol. Wende/Fong/Duncan. Kelly Lab. Revised May 29, 2007. DAY ONE (for cells): ... Keep at RT until protocol directs to put on ice. ...
kellylab.wustl.edu/protocols/ChIP_Protocol5-29-07.pdf –
Chromatin Immunoprecipitation (ChIP) Assay Protocol
(ChIP) Assay Protocol. Can be prepared in advance and stored at -80º ... ChIP Protocol. Page 2 of 12. Updated 6/30/06 By Heather N. Witt ...
www.genomecenter.ucdavis.edu/farnham/pdf/FarnhamLabChIP%20Protocol.pdf
Chromatin Immunoprecipitation (ChIPs) Protocol for Tissues(Farnham Lab). This protocol is based upon protocols from Mark Biggin, Dave Allis and Richard ...
genomecenter.ucdavis.edu/farnham/farnham/protocols/tissues.html
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION (X-ChIP) PROTOCOL
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION. (X-ChIP) PROTOCOL. Cross-linking and Cell Harvesting. 1. Start with 2 large dishes when confluent (1x10 ...
www.cosmobio.co.jp/product/kiki/pdf/ACM_chip_protocol.pdf
Day-and-a-half and two-and-a-half day ChIP
http://blog-di-j.blogspot.com/2007/12/optimized-chip-protocols.html. The blog post will also contain the most recent version of this protocol. 1.1 Protocol ...
www.jeremiahfaith.com/blog_figs/new_protocol_v1.0.pdf
Original ChIP protocol. Jeremiah Faith. Bioinformatics Program.
www.jeremiahfaith.com/blog_figs/original_protocol.pdf
1 BISHOP LAB DMC1 CHIP PROTOCOL Note: This protocol is optimized ...
BISHOP LAB DMC1 CHIP PROTOCOL. Note: This protocol is optimized for meiotic yeast cells sporulating in liquid. medium. CROSS-LINKING ...
bishoplab.bsd.uchicago.edu/protocols/BishopLabDmc1pChIP.pdf
ChIP Assay Protocol. By Michelle Kallesen, PhD (7/01). Formaldehyde cross-linking and chromatin immunoprecipitation assays of ...
www.bcm.edu/rosenlab/protocols/ChIP.pdf
Magnetic beads enable a streamlined ChIP method | Protocol Guide ...
We have developed a greatly simplified ChIP protocol that utilizes magnetic protein G-coated beads for antibody capture. This dramatically reduces washing ...
www.biotechniques.com/default.asp?page=protocol&subsection=article_display&id=112342
Steven Vokes McMahon Laboratory EB ChIP Protocol
EB ChIP Protocol. This protocol is a combination of Odom and colleagues (Odom et al., 2004) with a. Maniatis lab protocol (Daniel Smith and Diana Saville ...
www.people.fas.harvard.edu/~svokes/Vokes_ChIP_EB.pdf
1 ChIP protocol for procyclic forms 1. grow cells to a density of ...
ChIP protocol for procyclic forms. 1. grow cells to a density of 2 x 10. 7. cells/ml in SDM-79. 2. spin down (2000g for 10 min) and resuspend in 40 ml SDM- ...
tryps.rockefeller.edu/Protocols/ChIP_cj.pdf
Chromatin immunoprecipitation (ChIP) protocol from Drosophila embryos
Chromatin immunoprecipitation (ChIP) protocol from. Drosophila embryos. Overview. Target DNA binding protein that has been cross-linked to chromatin (which ...
www.flychip.org.uk/protocols/chip/purification_chromatin.pdf
Mapping DNA target sites of chromatin-associated proteins by ...
1. Reference: Cavalli, G., orlando, V and Paro, R. (1999). Mapping DNA target sites of chromatin-. associated proteins by formaldehyde cross-linking in ...
www.igh.cnrs.fr/equip/cavalli/Lab%20Protocols/FormaldehydeCrosslinking.pdf -
OneDay ChIP Kit—Product Manual
OneDay ChIP Kit Results. Troubleshooting Guide. Additional Protocols (Chromatin Shearing methods). References. Ordering Information ...
www.nippongene.com/pdf/man_oneday_english070405.pdf
DNA methylation detection using ChIP-GLAS
This short protocol is for the preparation of tissue samples that will be used in. ASB’s ChIP-GLAS system. You may use it to replace the corresponding part ...
www.avivasysbio.com/corp/Tissue%20ChIP%20protocol.PDF
Biochemistry and Molecular Genetics - UCHSC--ChIP for Yeast
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST. This protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. ...
www.uchsc.edu/sm/bbgn/ChIP_Protocol.htm
Chromatin Immunoprecipitation (ChIP) Methods, Techniques, Protocols
ChIP Protocol for Yeast - This protocol is derived from a paper by Miriam ... Chromatin IP (CHIP assay) - This protocol has some minor modification to the ...
www.antibodybeyond.com/applications/chip.htm
chromatin immunoprecipitation (ChIP) DNA. However, this method requires ..... Also see the troubleshooting guide of your ChIP preparation protocol or kit to ...
www.biomol.de/details/SA/chipqpcrmanual.pdf
Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from ...
Here, we describe how ChIP can be performed on non-fixed chromatin from animal ..... See Troubleshooting. DNA Extraction from Precipitated Chromatin (3 h) ...
www.cshprotocols.org/cgi/content/full/2007/12/pdb.prot4767
Sugden Lab. Last Modified 05/03/05 ChIP Protocol for 05/01/05-05 ...
ChIP Protocol for 05/01/05-05/03/05. Samples: Total, No Ab, IgG, E2F4, EBNA1, ORC2, ORC3. Sun 05/01/05. 1. Thaw 2 100ul-aliquots of Staph A cells (stored in ...
mcardle.oncology.wisc.edu/sugden/protocols/ChIP%20assay.pdf
Chromatin Immunoprecipation (ChIP) assay for 3T3-L1 preadipocytes
Chromatin Immunoprecipation (ChIP) assay for 3T3-L1 preadipocytes ... More troubleshooting will be required. But use this as a starting pointÉ ...
www-personal.umich.edu/~macdouga/Protocols/Chromatin%20Immunoprecipation.htm
next step of the protocol. The ChIP-IT Express Kit includes all .... In addition, the simplified ChIP-IT Express protocol enabled ChIP to be performed ...
www.biolynx.ca/Tech/Articles/ChIP%20protocol.pdf
Chromatin IP Chromatin Immunoprecipitation ChIP-IT
Using our chromatin and ChIP-IT reagents, the only variable in your ChIP will be the ... ChIP-IT Kits, simplify the task of troubleshooting your experiment, ...
www.activemotif.com/catalog/nuc_func/chipit/express
The principle of ChIP is simple: the selective enrichment of a chromatin fraction ... which we hope will help you troubleshoot this powerful method. ...
www.abcam.com/index.html?rid=310
More ChIP Tips (November 2005) | Abcam
Buying and contact info. Choose country,
www.abcam.com/index.html?pageconfig=resource&rid=10347&pid=5
troubleshooting with Chromatin Ip
troubleshooting with Chromatin Ip. Shwetal Mehta shwetal at mail.utexas.edu ... Hi, I have been having this problem with my ChIP assays (chromatin IP) of ...
www.bio.net/hypermail/yeast/2002-March/010125.html
Table 1 : Protocol for the fast chromatin immunoprecipitation ...--Troubleshooting
Protocol for the fast chromatin immunoprecipitation (ChIP) method. Joel D Nelson, Oleg Denisenko and Karol Bomsztyk ... Table 1. Troubleshooting table ...
www.nature.com/nprot/journal/v1/n1/fig_tab/nprot.2006.27_T1.html
The Science Advisory Board - Protocols, Product Reviews, Member ...
Yeast Chromatin Immunoprecipitation. Posted on Tuesday, November 18, 2003 ... Resuspend cell pellets in 400 ul ChIP lysis buffer+protease inhibitors ...
www.scienceboard.net/resources/protocols.asp?action=article&protocol_id=223&criteria
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION (X-ChIP) PROTOCOL
CROSS-LINKING CHROMATIN IMMUNOPRECIPITATION. (X-ChIP) PROTOCOL ... Reverse cross-links by adding either 2µl RNase A (0.5mg/ml) when purifying DNA using ...
www.cosmobio.co.jp/product/kiki/pdf/ACM_chip_protocol.pdf
Chromatin Immunoprecipitation (ChIP) Assay Protocol. (Double Cross
www.bioinfo.utmb.edu/Brasier_Lab/protocols/ChIP.pdf
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